Наборы антител BD Bioscience
Since the introduction of the firefly luciferase gene to molecular biology in 1986,1, 2 it has provided a method of utilizing biological light production as a tool for research. The luciferase gene product has a unique capability to interact with its cognate substrate luciferin and to produce a blue/green light photon, and so provides for an unparalleled controllable signal. For use in the laboratory, this form of light emission can yield a very sensitive nonradioactive assay.3-8 Firefly luciferase can be reliably expressed as a reporter gene from many expression vectors and in highly diverse organisms. Because of this ability, its major use has been to characterize gene regulation, primarily transcriptional control. By providing faster results, lower costs and over a 1,000-fold increase in sensitivity, the luciferase assay has largely replaced the standard 14C chloramphenicolacetyltransferase (CAT) assay
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